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Phospho-Tau-S404 Rabbit pAb (AP0170)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from SH-SY5Y and C6 cells, using Phospho-Tau-S404 Rabbit pAb (AP0170) at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100nM) for 1 hour and Calyculin A (100nM) for 30 minutes after serum-starvation overnight.C6 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - Phospho-Tau-S404 Rabbit pAb (AP0170)

Immunohistochemistry analysis of paraffin-embedded Rat hippocampal region tissue from a model with Alzheimer, using Phospho-Tau-S404 Rabbit pAb (AP0170). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-Tau-S404 Rabbit pAb (AP0170)

Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-Tau-S404 Rabbit pAb (AP0170).

Overview

Product name	Phospho-Tau-S404 Rabbit pAb
Catalog No.	AP0170
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S404 of human Tau (NP_005901.2).
Sequence	DTSPR
Gene ID
Swiss Prot
Synonyms	TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6; Phospho-Tau-S404
Calculated MW	79kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:100 IF/ICC 1:100 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	SH-SY5Y treated by Okadaic Acid and Calyculin A, C6
Cellular location	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, cytoskeleton, cytosol.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Mus musculus) IHC(Mus musculus)

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Phospho-Tau-S404 Rabbit pAb images

Western blot - Phospho-Tau-S404 Rabbit pAb (AP0170)

Immunohistochemistry - Phospho-Tau-S404 Rabbit pAb (AP0170)

Immunofluorescence - Phospho-Tau-S404 Rabbit pAb (AP0170)

Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-Tau-S404 Rabbit pAb (AP0170).

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Brain Research Bulletin

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32272155

Title:

Morin exerts protective effects on encephalopathy and sepsis-associated cognitive functions in a murine sepsis model

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Biomedicine & Pharmacotherapy

Application:

IF:

6.529

Species:

Mus musculus

PMID:

33197761

Title:

NaoXinTong Capsule ameliorates memory deficit in APP/PS1 mice by regulating inflammatory cytokines

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Food Chem Toxicol

Application:

IF:

6.02

Species:

Mus musculus

PMID:

34411643

Title:

Inhibition of sEH via stabilizing the level of EETs alleviated Alzheimer's disease through GSK3β signaling pathway

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

5.34

Species:

Mus musculus

PMID:

34607163

Title:

Gardenia jasminoides J. Ellis extract GJ-4 alleviated cognitive deficits of APP/PS1 transgenic mice

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Neuropharmacology

Application:

IF:

5.25

Species:

Mus musculus

PMID:

34543632

Title:

Low molecular weight chondroitin sulfate ameliorates pathological changes in 5XFAD mice by improving various functions in the brain

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

ACS chemical neuroscience

Application:

IF:

5.78

Species:

Homo sapiens

PMID:

36350059

Title:

Site-Specific Phospho-Tau Aggregation-Based Biomarker Discovery for AD Diagnosis and Differentiation

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Acta neuropathologica communications

Application:

IF:

7.57

Species:

Mus musculus

PMID:

37024941

Title:

Exogenous interleukin 33 enhances the brains lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice - PMC

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Front Aging Neurosci

Application:

WB, IF

IF:

5.702

Species:

Mus musculus

PMID:

33328962

Title:

LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

Rattus norvegicus

PMID:

37910285

Title:

Schisandrin A Alleviates Spatial Learning and Memory Impairment in Diabetic Rats by Inhibiting Inflammatory Response and Through Modulation of the PI3K/AKT

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Journal of neurotrauma

Application:

IF:

4.2

Species:

Mus musculus

PMID:

38553903

Title:

Cannabidiol alleviates neurological deficits after traumatic brain injury by improving intracranial lymphatic drainage

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IHC

IF:

14.5

Species:

Mus musculus

PMID:

35024324

Title:

A nanocleaner specifically penetrates the bloodbrain barrier at lesions to clean toxic proteins and regulate inflammation in Alzheimer's disease

References for Phospho-Tau-S404 Rabbit pAb (AP0170)

Product:

Phospho-Tau-S404 Rabbit pAb

Journal:

Journal of Alzheimer's disease : JAD

Application:

IF:

Species:

Homo sapiens

PMID:

39213070

Title:

Identification of the Shared Gene Signatures Between Alzheimers Disease and Diabetes-Associated Cognitive Dysfunction by Bioinformatics Analysis Combined with Biological Experiment - PMC