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Phospho-Tau (S214) Rabbit mAb (AP1423)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis lysates from SH-SY5Y, using Phospho-Tau (S214) Rabbit mAb (AP1423) at 1:1000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100 nM) at 37℃ for 1 hour and Calyculin A (100nM) at 37℃ for 30 minutes after serum-starvation overnight.℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-Tau (S214) Rabbit mAb (AP1423)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue, (5XFAD)Mouse brain (left) and normal mouse brain (right), using Phospho-Tau (S214) Rabbit mAb (AP1423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-Tau (S214) Rabbit mAb
Catalog No.	AP1423
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3098

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Immunogen information

Immunogen	A phospho synthetic peptide corresponding to residues surrounding S214 of Human Tau.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; tau-40; FTDP-17; PPP1R103; Tau-PHF6; Phospho-Tau (S214)
Calculated MW	79kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	SH-SY5Y treated by Okadaic Acid and Calyculin A
Cellular location	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, axon, cytoskeleton, cytosol
Customer validation	WB(Mus musculus)