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[KO Validated] Bax Rabbit mAb (A18642)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit mAb (A18642) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from HT-1080 cells, using [KO Validated] Bax Rabbit mAb (A18642) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A18642)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] Bax Rabbit mAb (A18642) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Bax Rabbit mAb (A18642) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit mAb (A18642)

Confocal imaging of HeLa cells using [KO Validated] Bax Rabbit mAb (A18642, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] Bax Rabbit mAb (A18642)

Immunoprecipitation analysis of 200 μg extracts of 293T using 3 μg Bax antibody (A18642). Western blot was performed from the immunoprecipitate using Bax antibody (A18642) at a dilution of 1:1000.

Overview

Product name	[KO Validated] Bax Rabbit mAb
Catalog No.	A18642
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5006-06

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1).
Sequence	GGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDEL
Gene ID
Swiss Prot
Synonyms	BCL2L4; ax
Calculated MW	21kDa
Observed MW	21kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:2000 - 1:8000 IF/ICC 1:200 - 1:800 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, HT-1080
Cellular location	Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Oreochromis niloticus, Rattus norvegicus) IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Bax Rabbit mAb images

Western blot - [KO Validated] Bax Rabbit mAb (A18642)

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A18642)

Immunofluorescence - [KO Validated] Bax Rabbit mAb (A18642)

Immunoprecipitation - [KO Validated] Bax Rabbit mAb (A18642)

Immunoprecipitation analysis of 200 μg extracts of 293T using 3 μg Bax antibody (A18642). Western blot was performed from the immunoprecipitate using Bax antibody (A18642) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Homo sapiens

PMID:

33679411

Title:

Isorhamnetin Inhibits Human Gallbladder Cancer Cell Proliferation and Metastasis via PI3K/AKT Signaling Pathway Inactivation.

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Mus musculus

PMID:

35676786

Title:

The whole transcriptome analysis and the circRNA-lncRNA network construction in arsenic trioxide-treated mice myocardium

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Aquatic toxicology (Amsterdam, Netherlands)

Application:

IHC

IF:

5.2

Species:

Oreochromis niloticus

PMID:

35933907

Title:

Appraisal of sub-chronic exposure to lambada-cyhalothrin and/or methomyl on the behavior and hepato-renal functioning in Oreochromis niloticus: Supportive role of …

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Open medicine (Warsaw, Poland)

Application:

IF:

Species:

Homo sapiens

PMID:

36941991

Title:

Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9 - PMC

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Biomedicines

Application:

IF:

4.75

Species:

Homo sapiens

PMID:

38275415

Title:

Identification of a Novel Germline PPP4R3A Missense Mutation Asp409Asn on Familial Non-Medullary Thyroid Carcinoma - PMC

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

7.4

Species:

Homo sapiens,Mus musculus

PMID:

38461872

Title:

The long coiled-coil protein NECC2 regulates oxLDL-induced endothelial oxidative damage and exacerbates atherosclerosis development in apolipoprotein E

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Adipocyte

Application:

WB, IHC

IF:

3.55

Species:

Homo sapiens

PMID:

37993991

Title:

Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Renal failure

Application:

IF:

Species:

Mus musculus

PMID:

38856314

Title:

H-151 attenuates lipopolysaccharide-induced acute kidney injury by inhibiting the STING-TBK1 pathway - PMC

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Toxicology mechanisms and methods

Application:

IHC

IF:

3.2

Species:

Rattus norvegicus

PMID:

38845370

Title:

Multiple-heated cooking oil promotes early hepatic and renal senescence in adult male rats: The potential regenerative capacity of oleuropein.

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Mus musculus

PMID:

39380664

Title:

Mangiferin Ameliorates CCl4-Triggered Acute Liver Injury by Inhibiting Inflammatory Response and Oxidative Stress: Involving the Nrf2-ARE Pathway - PMC

References for [KO Validated] Bax Rabbit mAb (A18642)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

39032406