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[KO Validated] Bax Rabbit mAb (A20227)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using [KO Validated] Bax Rabbit mAb (A20227) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from wild type(WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit mAb (A20227) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KO Validated] Bax Rabbit mAb (A20227)

Immunofluorescence analysis of C6 cells using Bax Rabbit mAb (A20227) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Bax Rabbit mAb (A20227) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Bax Rabbit mAb (A20227) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Bax Rabbit mAb (A20227)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Bax antibody (A20227). Western blot was performed from the immunoprecipitate using Bax antibody (A20227) at a dilution of 1:500.

Overview

Product name	[KO Validated] Bax Rabbit mAb
Catalog No.	A20227
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5006-10

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1).
Sequence	GGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDEL
Gene ID
Swiss Prot
Synonyms	BCL2L4; ax
Calculated MW	21kDa
Observed MW	21kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IF/ICC 1:100 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, HT-1080, RAW264.7
Cellular location	Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Customer validation	WB(Rattus norvegicus, Mus musculus, Capra hircus) IF(Mus musculus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Bax Rabbit mAb images

Western blot - [KO Validated] Bax Rabbit mAb (A20227)

Immunofluorescence - [KO Validated] Bax Rabbit mAb (A20227)

Immunoprecipitation - [KO Validated] Bax Rabbit mAb (A20227)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Rattus norvegicus

PMID:

34280397

Title:

Thymosin beta 4 alleviates non-alcoholic fatty liver by inhibiting ferroptosis via up-regulation of GPX4 0

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

36786681

Title:

Confusoside, a dihydrochalcone glucoside, prevents acetaminophen-induced liver injury by modulating the Keap1-Nrf2/Nf-κB/Caspase signaling pathway

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Current issues in molecular biology

Application:

IF:

2.97

Species:

Mus musculus

PMID:

37998720

Title:

Implications of Activating the ANT2/mTOR/PGC-1 Feedback Loop: Insights into Mitochondria-Mediated Injury in Hypoxic Myocardial Cells - PMC

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

5.7

Species:

Mus musculus

PMID:

38020765

Title:

CCL5 promotes LFA-1 expression in Th17 cells and induces LCK and ZAP70 activation in a mouse model of Parkinsons disease - PMC

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

5.7

Species:

Mus musculus

PMID:

38020765

Title:

CCL5 promotes LFA-1 expression in Th17 cells and induces LCK and ZAP70 activation in a mouse model of Parkinsons disease - PMC

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Mus musculus

PMID:

37951147

Title:

Anticancer activity of Bacopa monnieri through apoptosis induction and mitophagy-dependent NLRP3 inflammasome inhibition in oral squamous cell carcinoma

References for [KO Validated] Bax Rabbit mAb (A20227)

Product:

[KO Validated] Bax Rabbit mAb

Journal:

International journal of molecular medicine

Application:

WB, IF

IF:

5.31

Species:

Rattus norvegicus

PMID: