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[KO Validated] Bax Rabbit mAb (A19684)

KO/KDValidated

Publications (120) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of various lysates using Bax Rabbit mAb (A19684) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using Bax Rabbit mAb (A19684) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name[KO Validated] Bax Rabbit mAb
Catalog No.A19684
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0164
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (Q07812).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID
Swiss Prot
SynonymsBCL2L4; Bax
Calculated MW21kDa
Observed MW21kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:1000 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Raji, RAW 264.7
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Customer validation

WB(Mus musculus, Sanghuangporus vaninii, Rattus norvegicus, Homo sapiens, Sus scrofa , Homo sapiens, Fathead minnow, Dianella ensifolia, Rattus norvegicus, Mus musculus, Other, Ctenopharyngodon idellus)

IF(Homo sapiens)

WB(Homo sapiens)

IHC(Rattus norvegicus)

IHC(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)}

Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of various lysates using Bax Rabbit mAb (A19684) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] Bax Rabbit mAb (A19684)}

Western blot - [KO Validated] Bax Rabbit mAb (A19684)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using Bax Rabbit mAb (A19684) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)}

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)}

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)}

Immunohistochemistry - [KO Validated] Bax Rabbit mAb (A19684)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Bax Rabbit mAb (A19684) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19684? Please let us know so that we can cite the reference in this datasheet.

항체 (5)

ELISA 키트 (1)

Secondary Antibodies (26)