제품 > 항체 > 단일클론항체(mAb)

[KO Validated] ATG5 Rabbit mAb (A19677)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells, using [KO Validated] ATG5 Rabbit mAb (A19677) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of various lysates using [KO Validated] ATG5 Rabbit mAb (A19677) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] ATG5 Rabbit mAb (A19677)

Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb (A19677, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb (A19677, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KO Validated] ATG5 Rabbit mAb (A19677). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:1000.

Review

Overview

Product name	[KO Validated] ATG5 Rabbit mAb
Catalog No.	A19677
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0156

Background

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0).
Sequence	MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNI
Gene ID
Swiss Prot
Synonyms	ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE; G5
Calculated MW	32kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IHC-P 1:200 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	293T, HeLa, SH-SY5Y, Mouse brain, Mouse lung
Cellular location	Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane.
Customer validation	(293T, Hela,A549,HepG2,PC-3,HL-60,RPMI-8226,MLOT-4 , 293T, Hela, A549, HepG2, PC-3, HL-60, RPMI-8226, MOLT-4) WB(Spodoptera frugiperda, Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Other, Oryctolagus cuniculus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구세포 생물학 & 발달 생물학자가포식ATGs

[KO Validated] ATG5 Rabbit mAb images

Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunofluorescence - [KO Validated] ATG5 Rabbit mAb (A19677)

Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A19677

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Reviews List

Product Protocols

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Virulence

Application:

IF:

5.5

Species:

Spodoptera frugiperda

PMID:

33509041

Title:

Autophagy induced by Vip3Aa has a pro-survival role in Spodoptera frugiperda Sf9 cells

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Exp Mol Pathol

Application:

IF:

2.8

Species:

Mus musculus

PMID:

32888957

Title:

Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Autophagy

Application:

IF:

14.6

Species:

Homo sapiens

PMID:

36048765

Title:

Isoginkgetin, a Potential CDK6 Inhibitor, Suppresses SLC2A1/GLUT1 Enhancer Activity to Induce AMPK-ULK1-Mediated Cytotoxic Autophagy in Hepatocellular …

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Asian journal of pharmaceutical sciences

Application:

IF:

10.7

Species:

Homo sapiens

PMID:

36891470

Title:

Selective ischemic-hemisphere targeting Ginkgolide B liposomes with improved solubility and therapeutic efficacy for cerebral ischemia-reperfusion injury - PMC

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Psychopharmacology

Application:

IF:

3.5

Species:

Rattus norvegicus

PMID:

36949340

Title:

Curcumin protects against doxorubicin induced oxidative stress by regulating the Keap1-Nrf2-ARE and autophagy signaling pathways

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Cellular and molecular life sciences : CMLS

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

37183204

Title:

Development of VHL-recruiting STING PROTACs that suppress innate immunity

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Rattus norvegicus

PMID:

37447299

Title:

Piceatannol Protects PC-12 Cells against Oxidative Damage and Mitochondrial Dysfunction by Inhibiting Autophagy via SIRT3 Pathway - PMC

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Journal of virology

Application:

IF:

Species:

Gallus gallus

PMID:

37255472

Title:

Critical Role of Viral Protein Hexon in Hypervirulent Fowl Adenovirus Serotype-4-Induced Autophagy by Interaction with BAG3 and Promotion of Viral Replication in …

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Journal of virology

Application:

IF:

Species:

Gallus gallus

PMID:

37255472

Title:

Critical Role of Viral Protein Hexon in Hypervirulent Fowl Adenovirus Serotype-4-Induced Autophagy by Interaction with BAG3 and Promotion of Viral Replication in …

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Cell reports

Application:

IF:

7.5

Species:

Mus musculus

PMID:

36198274

Title:

Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Bioscience reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

37103469

Title:

Identification of PPT1 as a lysosomal core gene with prognostic value in hepatocellular carcinoma

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

Species:

Homo sapiens

PMID:

35795282

Title:

Isoschaftoside Reverses Nonalcoholic Fatty Liver Disease via Activating Autophagy In Vivo and In Vitro - PMC

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

IUBMB life

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

37818680

Title:

Nacetylneuraminic acid modulates SQSTM1/p62 sialyationmediated ubiquitination degradation contributing to vascular endothelium dysfunction in experimental

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

EMBO reports

Application:

IF:

6.5

Species:

Homo sapiens

PMID:

37846507

Title:

An ULK1/2PXN mechanotransduction pathway suppresses breast cancer cell migration

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Environmental toxicology

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38095090

Title:

Curcumin attenuates diphenyl phosphateinduced apoptosis in GC2spd (ts) cells through activated autophagy via the Nrf2/P53 pathway

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Rattus norvegicus

PMID:

36808838

Title:

Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a MetabolismEpigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in

References for [KO Validated] ATG5 Rabbit mAb (A19677)

Product:

[KO Validated] ATG5 Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Other

PMID:

38538581