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[KO Validated] ATG5 Rabbit pAb (A0203)

KO/KDValidated

Reviews (6)Publications (56) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)

Western blot analysis of various lysates using ATG5 Rabbit pAb (A0203) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)

Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells using ATG5 Rabbit pAb (A0203) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Human colon using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunofluorescence analysis of K-562 cells using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for [KO Validated] ATG5 Rabbit pAb(A0203)

Overview

Product name[KO Validated] ATG5 Rabbit pAb
Catalog No.A0203
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1).
SequenceMTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
Gene ID
Swiss Prot
SynonymsASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE; ATG5
Calculated MW32kDa
Observed MW55kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, NIH/3T3, K-562, C6, SH-SY5Y, 293T
Cellular locationCytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane.
Customer validation

(Human chronic myeloid leukemia cells, Human colon cancer cell, H460, Mouse lung tumor, A549,Mouse cells, HeLa)

WB(Sus scrofa, Gallus gallus, Columba livia, Homo sapiens, Rattus norvegicus, Mus musculus, Anatinae, Other, Bos taurus, Danio rerio)

IF(Sus scrofa, Homo sapiens, Mus musculus)

IHC(Mus musculus)

IF(Other, Bos taurus, Mus musculus)

IHC(Mus musculus, Bos taurus)

ELISA(Mus musculus)

IP(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)}

Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)

Western blot analysis of various lysates using ATG5 Rabbit pAb (A0203) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)}

Western blot - [KO Validated] ATG5 Rabbit pAb (A0203)

Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells using ATG5 Rabbit pAb (A0203) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)}

Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)}

Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)}

Immunohistochemistry - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunohistochemistry analysis of paraffin-embedded Human colon using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] ATG5 Rabbit pAb (A0203)}

Immunofluorescence - [KO Validated] ATG5 Rabbit pAb (A0203)

Immunofluorescence analysis of K-562 cells using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (2)

Secondary Antibodies (26)