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FAK Rabbit pAb (A2114)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FAK Rabbit pAb (A2114)

Western blot analysis of lysates from U-87MG cells, using FAK Rabbit pAb (A2114) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - FAK Rabbit pAb (A2114)

Immunohistochemistry analysis of paraffin-embedded human esophagus using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FAK Rabbit pAb (A2114)

Immunohistochemistry analysis of paraffin-embedded rat kidney using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - FAK Rabbit pAb (A2114)

Immunofluorescence analysis of C6 cells using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FAK Rabbit pAb (A2114)

Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFAK Rabbit pAb
Catalog No.A2114
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (NP_722560.1).
SequenceTVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIG
Gene ID
Swiss Prot
SynonymsFAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK
Calculated MW119kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - FAK Rabbit pAb (A2114)}

Western blot - FAK Rabbit pAb (A2114)

Western blot analysis of lysates from U-87MG cells, using FAK Rabbit pAb (A2114) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - FAK Rabbit pAb (A2114)}

Immunohistochemistry - FAK Rabbit pAb (A2114)

Immunohistochemistry analysis of paraffin-embedded human esophagus using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FAK Rabbit pAb (A2114)}

Immunohistochemistry - FAK Rabbit pAb (A2114)

Immunohistochemistry analysis of paraffin-embedded rat kidney using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - FAK Rabbit pAb (A2114)}

Immunofluorescence - FAK Rabbit pAb (A2114)

Immunofluorescence analysis of C6 cells using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FAK Rabbit pAb (A2114)}

Immunofluorescence - FAK Rabbit pAb (A2114)

Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit pAb (A2114) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)