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FAM3D Rabbit pAb (A17824)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FAM3D Rabbit pAb (A17824)

Western blot analysis of various lysates using FAM3D Rabbit pAb (A17824) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (20x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)

Immunohistochemistry analysis of paraffin-embedded Human colon using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - FAM3D Rabbit pAb (A17824)

Immunofluorescence analysis of L929 cells using FAM3D Rabbit pAb (A17824) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFAM3D Rabbit pAb
Catalog No.A17824
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in negative regulation of insulin secretion. Predicted to be located in extracellular region. Predicted to be integral component of membrane. Predicted to be active in extracellular space.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-204 of human FAM3D (NP_620160.1).
SequenceRSYMSFSMKTIRLPRWLAASPTKEIQVKKYKCGLIKPCPANYFAFKICSGAANVVGPTMCFEDRMIMSPVKNNVGRGLNIALVNGTTGAVLGQKAFDMYSGDVMHLVKFLKEIPGGALVLVASYDDPGTKMNDESRKLFSDLGSSYAKQLGFRDSWVFIGAKDLRGKSPFEQFLKNSPDTN
Gene ID
Swiss Prot
SynonymsEF7; OIT1; FAM3D
Calculated MW25kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, Mouse large intestine
Cellular locationextracellular region, extracellular space

Documents

Certificate of Compliance

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    ABclonal:Western blot - FAM3D Rabbit pAb (A17824)}

    Western blot - FAM3D Rabbit pAb (A17824)

    Western blot analysis of various lysates using FAM3D Rabbit pAb (A17824) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)}

    Immunohistochemistry - FAM3D Rabbit pAb (A17824)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (20x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)}

    Immunohistochemistry - FAM3D Rabbit pAb (A17824)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - FAM3D Rabbit pAb (A17824)}

    Immunohistochemistry - FAM3D Rabbit pAb (A17824)

    Immunohistochemistry analysis of paraffin-embedded Human colon using FAM3D Rabbit pAb (A17824) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - FAM3D Rabbit pAb (A17824)}

    Immunofluorescence - FAM3D Rabbit pAb (A17824)

    Immunofluorescence analysis of L929 cells using FAM3D Rabbit pAb (A17824) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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