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FEN-1 Rabbit mAb (A8999)

Review (2)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - FEN-1 Rabbit mAb (A8999)

Western blot analysis of various lysates using FEN-1 Rabbit mAb (A8999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - FEN-1 Rabbit mAb (A8999)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FEN-1 Rabbit mAb (A8999) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Review
ABclonal: review for FEN-1 Rabbit mAb(A8999)
Review
ABclonal: review for FEN-1 Rabbit mAb(A8999)

Overview

Product nameFEN-1 Rabbit mAb
Catalog No.A8999
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1376
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748).
SequenceIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSL
Gene ID
Swiss Prot
SynonymsMF1; RAD2; FEN-1
Calculated MW43kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, HUVEC, Mouse thymus
Cellular locationMitochondrion, Nucleus, nucleolus, nucleoplasm
Customer validation

(HT29 、K562 、U2-OS, HT29 K562 U2-OS)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - FEN-1 Rabbit mAb (A8999)}

Western blot - FEN-1 Rabbit mAb (A8999)

Western blot analysis of various lysates using FEN-1 Rabbit mAb (A8999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - FEN-1 Rabbit mAb (A8999)}

Immunohistochemistry - FEN-1 Rabbit mAb (A8999)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FEN-1 Rabbit mAb (A8999) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)