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FGFR1 Rabbit mAb (A21219)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FGFR1 Rabbit mAb (A21219)

Western blot analysis of lysates from U-251MG cells, using FGFR1 Rabbit mAb (A21219) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - FGFR1 Rabbit mAb (A21219)

Western blot analysis of various lysates, using FGFR1 Rabbit mAb (A21219) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - FGFR1 Rabbit mAb (A21219)

Confocal imaging of HeLa cells using FGFR1 Rabbit mAb (A21219, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameFGFR1 Rabbit mAb
Catalog No.A21219
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53424
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human FGFR1 (NP_075598.2).
SequenceLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSP
Gene ID
Swiss Prot
SynonymsCEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1; FGFR1
Calculated MW92kDa
Observed MW122kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG, Rat brain, Mouse brain
Cellular locationCell membrane, Cytoplasm, Cytoplasmic vesicle, Nucleus, Single-pass type I membrane protein, cytosol.
Customer validation

WB(Homo sapiens, Mus musculus)

Cell-based assay(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - FGFR1 Rabbit mAb (A21219)}

Western blot - FGFR1 Rabbit mAb (A21219)

Western blot analysis of lysates from U-251MG cells, using FGFR1 Rabbit mAb (A21219) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - FGFR1 Rabbit mAb (A21219)}

Western blot - FGFR1 Rabbit mAb (A21219)

Western blot analysis of various lysates, using FGFR1 Rabbit mAb (A21219) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FGFR1 Rabbit mAb (A21219)}

Immunohistochemistry - FGFR1 Rabbit mAb (A21219)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using FGFR1 Rabbit mAb (A21219) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - FGFR1 Rabbit mAb (A21219)}

Immunofluorescence - FGFR1 Rabbit mAb (A21219)

Confocal imaging of HeLa cells using FGFR1 Rabbit mAb (A21219, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21219? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)