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Phospho-FGFR1-Y653/Y654 Rabbit pAb (AP1317)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Phospho-FGFR1-Y653/Y654 Rabbit pAb (AP1317)

Western blot analysis of extracts of various cell lines, using Phospho-FGFR1-Y653/Y654 antibody (AP1317) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePhospho-FGFR1-Y653/Y654 Rabbit pAb
Catalog No.AP1317
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
ImmunogenA synthetic phosphorylated peptide around Y653 & Y654 of human Phospho-FGFR1-Y653/Y654 (NP_075598.2).
SequenceIDYYKK
Gene ID
Swiss Prot
SynonymsCEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Calculated MW92kDa
Observed MW120kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3
Cellular locationcytoplasmic vesicle, cytosol, extracellular region, nucleus, plasma membrane
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - Phospho-FGFR1-Y653/Y654 Rabbit pAb (AP1317)}

Western blot - Phospho-FGFR1-Y653/Y654 Rabbit pAb (AP1317)

Western blot analysis of extracts of various cell lines, using Phospho-FGFR1-Y653/Y654 antibody (AP1317) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP1317? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)