제품 > 항체 > 다중클론항체(pAb)

FOXP2 Rabbit pAb (A5677)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FOXP2 Rabbit pAb (A5677)

Western blot analysis of various lysates using FOXP2 Rabbit pAb (A5677) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameFOXP2 Rabbit pAb
Catalog No.A5677
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Multiple alternative transcripts encoding different isoforms have been identified in this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 590-715 of human FOXP2 (NP_055306.1).
SequenceGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLLSNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAEDEDCPMSLVTTANHSPELEDDREIEEEPLSEDLE
Gene ID
Swiss Prot
SynonymsSPCH1; CAGH44; TNRC10; FOXP2
Calculated MW80kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, A-549, SH-SY5Y, SGC-7901, Mouse lung, Mouse brain,Mouse stomach,Rat brain
Cellular locationNucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - FOXP2 Rabbit pAb (A5677)}

Western blot - FOXP2 Rabbit pAb (A5677)

Western blot analysis of various lysates using FOXP2 Rabbit pAb (A5677) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5677? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)