제품 > 항체 > 다중클론항체(pAb)

FTO Rabbit pAb (A18672)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using FTO Rabbit pAb (A18672) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	FTO Rabbit pAb
Catalog No.	A18672
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable 2-oxoglutarate-dependent dioxygenase activity and ferrous iron binding activity. Acts upstream of or within several processes, including cilium assembly; left/right pattern formation; and positive regulation of canonical Wnt signaling pathway. Predicted to be located in cytoplasm and nuclear speck. Human ortholog(s) of this gene implicated in obesity. Orthologous to human FTO (FTO alpha-ketoglutarate dependent dioxygenase).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of zebrafish FTO (XP_009301626.1).
Sequence	MKPRQRKQYFRNMKRSDDSEREKRRKRRRLLQELGEQRIPYLSPTDPGFQDLWDSSYAGLALRQSGTLPEGLHEKVQSALLTLQRHGCLLRDLVRVRDRDVFTAVSRALVGQPGYTYRYLDTRLFTIPWHCEGEEGQKDEKGKPCCDSDLRDACKALWELNQFFCSDVKQQTNARGVKRTRSDTENSEDAPGEGMCEEESVKDRLVEEKTIEEEEEDSGQGCSHSSPPSSTPPAAQASLVQFNVTLINYMNPAAMTQLKEEPYYGMGKMAVGWHHDENLVPLSPVAVYSYSCPAEPKNEGVTEKDGEGKSKEKEREAKGEGTSTEEVKKEGEASVKEDVEKEKTCWRVG
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse brain, Rat brain
Cellular location