Western blot - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Western blot analysis of various lysates using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at 1:12000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Mouse brain
Exposure time: 90s.
Immunohistochemistry - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146) at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Confocal imaging of paraffin-embedded Human skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146)
Confocal imaging of paraffin-embedded Rat skeletal muscle tissue using Fast Myosin Skeletal Heavy chain Rabbit mAb (A27146, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.