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MYH1 Rabbit pAb (A6935)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MYH1 Rabbit pAb (A6935)

Western blot analysis of various lysates using MYH1 Rabbit pAb (A6935) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - MYH1 Rabbit pAb (A6935)

Western blot analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit pAb (A6935) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:3s.

ABclonal:Immunohistochemistry - MYH1 Rabbit pAb (A6935)

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using MYH1 Rabbit pAb (A6935) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded Human heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded rat heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMYH1 Rabbit pAb
Catalog No.A6935
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3).
SequenceMSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEP
Gene ID
Swiss Prot
SynonymsMYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D; MYH1
Calculated MW223kDa
Observed MW251kDa/250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse skeletal muscle, Rat skeletal muscle, Mouse skeletal muscle
Cellular locationCytoplasm, myofibril.
Customer validation

WB(Sus scrofa, Mus musculus)

(Homo sapiens)

IHC(Mus musculus)

IF(Scandentia, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MYH1 Rabbit pAb (A6935)}

Western blot - MYH1 Rabbit pAb (A6935)

Western blot analysis of various lysates using MYH1 Rabbit pAb (A6935) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - MYH1 Rabbit pAb (A6935)}

Western blot - MYH1 Rabbit pAb (A6935)

Western blot analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit pAb (A6935) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:3s.
ABclonal:Immunohistochemistry - MYH1 Rabbit pAb (A6935)}

Immunohistochemistry - MYH1 Rabbit pAb (A6935)

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using MYH1 Rabbit pAb (A6935) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)}

Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded Human heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)}

Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MYH1 Rabbit pAb (A6935)}

Immunofluorescence - MYH1 Rabbit pAb (A6935)

Immunofluorescence analysis of paraffin-embedded rat heart using MYH1 Rabbit pAb (A6935) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6935? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)