제품 > 항체 > 단일클론항체(mAb)

FceR1 alpha Rabbit mAb (A22729)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using FceR1 alpha Rabbit mAb (A22729) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	FceR1 alpha Rabbit mAb
Catalog No.	A22729
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58693

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-205 of human FceR1 alpha. (NP_001992.1).
Sequence	VPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQ
Gene ID
Swiss Prot
Synonyms	FCE1A; FcERI; FCERIA; FceR1 alpha
Calculated MW	30kDa
Observed MW	45-70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:7000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HEL, RAW264.7, Rat thymus
Cellular location	Cell membrane, Single-pass type I membrane protein
Customer validation	FC(Homo sapiens,Mus musculus) IF(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus)