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Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)

Western blot analysis of various lysates using Fibrinogen alpha chain (FGA) Rabbit pAb (A1453) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)

Immunohistochemistry analysis of paraffin-embedded human placenta using Fibrinogen alpha chain (Fibrinogen alpha chain (FGA)) Rabbit pAb (A1453) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameFibrinogen alpha chain (FGA) Rabbit pAb
Catalog No.A1453
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 410-559 of human Fibrinogen alpha chain (FGA) (NP_000499.1).
SequenceWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKE
Gene ID
Swiss Prot
SynonymsFib2; Fibrinogen alpha chain (FGA)
Calculated MW95kDa
Observed MW95kDa/60-70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse liver, Rat liver
Cellular locationSecreted
Customer validation

ELISA(Homo sapiens)

Documents

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ABclonal:Western blot - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)}

Western blot - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)

Western blot analysis of various lysates using Fibrinogen alpha chain (FGA) Rabbit pAb (A1453) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)}

Immunohistochemistry - Fibrinogen alpha chain (FGA) Rabbit pAb (A1453)

Immunohistochemistry analysis of paraffin-embedded human placenta using Fibrinogen alpha chain (Fibrinogen alpha chain (FGA)) Rabbit pAb (A1453) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1453? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)