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GABARAPL2 Rabbit mAb (A9585)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GABARAPL2 Rabbit mAb (A9585)

Western blot analysis of extracts of various cell lines, using GABARAPL2 Rabbit mAb (A9585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameGABARAPL2 Rabbit mAb
Catalog No.A9585
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1648
Enables ubiquitin protein ligase binding activity. Involved in negative regulation of proteasomal protein catabolic process and protein localization to endoplasmic reticulum. Located in Golgi membrane and autophagosome membrane.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 38-117 of human GABARAPL2 (P60520).
SequenceGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF
Gene ID
Swiss Prot
SynonymsATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16; FLC3A
Calculated MW14kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3, RD, Mouse brain, Rat brain
Cellular locationCytoplasmic vesicle, Golgi apparatus, autophagosome, Endoplasmic reticulum membrane
Customer validation

WB(Nicotiana tabacum)

Documents

Certificate of Compliance

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ABclonal:Western blot - GABARAPL2 Rabbit mAb (A9585)}

Western blot - GABARAPL2 Rabbit mAb (A9585)

Western blot analysis of extracts of various cell lines, using GABARAPL2 Rabbit mAb (A9585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)