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GABARAPL2 Rabbit pAb (A14855)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - GABARAPL2 Rabbit pAb (A14855)

Western blot analysis of various lysates using GABARAPL2 Rabbit pAb (A14855) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameGABARAPL2 Rabbit pAb
Catalog No.A14855
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ubiquitin protein ligase binding activity. Involved in negative regulation of proteasomal protein catabolic process and protein localization to endoplasmic reticulum. Located in Golgi membrane and autophagosome membrane.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-50 of human GABARAPL2 (NP_009216.1).
SequenceMKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYL
Gene ID
Swiss Prot
SynonymsATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16; GABARAPL2
Calculated MW14kDa
Observed MW17-18kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Raji, Mouse brain, Mouse thymus
Cellular locationCytoplasmic vesicle, Golgi apparatus, autophagosome

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ABclonal:Western blot - GABARAPL2 Rabbit pAb (A14855)}

Western blot - GABARAPL2 Rabbit pAb (A14855)

Western blot analysis of various lysates using GABARAPL2 Rabbit pAb (A14855) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

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