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GINS4 Rabbit pAb (A8592)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GINS4 Rabbit pAb (A8592)

Western blot analysis of extracts of various cell lines, using GINS4 antibody (A8592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameGINS4 Rabbit pAb
Catalog No.A8592
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 108-207 of human GINS4 (NP_115712.1).
SequenceLEKEKTRPEGEPSSLSPEELAFAREFMANTESYLKNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQENILVEPDTDEQRDYVIDLEKGSQHLIR
Gene ID
Swiss Prot
SynonymsSLD5
Calculated MW98kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, 293T, HeLa, THP-1, Mouse thymus, Rat fat
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - GINS4 Rabbit pAb (A8592)}

    Western blot - GINS4 Rabbit pAb (A8592)

    Western blot analysis of extracts of various cell lines, using GINS4 antibody (A8592) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8592? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)