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GL7 Rabbit pAb (A20641)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - GL7 Rabbit pAb (A20641)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GL7 Rabbit pAb (A20641) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameGL7 Rabbit pAb
Catalog No.A20641
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CTB4a (cold tolerance at booting stage), encoding a conserved leucine-rich repeat receptor-like kinase. The different CTB4a alleles confer distinct levels of cold tolerance and selection for variation in the CTB4a promoter region has occurred on the basis of environmental temperature.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 444-570 of Oryza sativa GL7. (Q6YW00).
SequenceHAKDAKRQNDGNGALTVAAQQAGDALNTSSRHFQPPIVVMKPARSTEKQPGVSLASVDPLAGFRNLRKLQARDAPCIGEHETSTNEKVHSRISRAQSKSDEPASRASSPRPTGSSSPRTVQRKAESE
Gene ID
Swiss Prot
SynonymsGL7
Calculated MW104kDa
Observed MW104kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesspikes
Cellular location
    ABclonal:Western blot - GL7 Rabbit pAb (A20641)}

    Western blot - GL7 Rabbit pAb (A20641)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GL7 Rabbit pAb (A20641) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)