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GLP-2 Rabbit mAb (A5009)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunofluorescence - GLP-2 Rabbit mAb (A5009)

Confocal imaging of paraffin-embedded Rat pancreas tissue using GLP-2 Rabbit mAb (A5009, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GLP-2 Rabbit mAb (A5009)

Confocal imaging of paraffin-embedded Mouse pancreas tissue using GLP-2 Rabbit mAb (A5009, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameGLP-2 Rabbit mAb
Catalog No.A5009
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1143
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 81-180 of human GLP-2 (P01275).
SequenceTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK
Gene ID
Swiss Prot
SynonymsGLP1; GLP2; GRPP; GLP-1; GLP-2
Calculated MW21kDa
Observed MW
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationSecreted
Customer validation

IHC(Mus musculus)

IF(Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Immunofluorescence - GLP-2 Rabbit mAb (A5009)}

Immunofluorescence - GLP-2 Rabbit mAb (A5009)

Confocal imaging of paraffin-embedded Rat pancreas tissue using GLP-2 Rabbit mAb (A5009, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GLP-2 Rabbit mAb (A5009)}

Immunofluorescence - GLP-2 Rabbit mAb (A5009)

Confocal imaging of paraffin-embedded Mouse pancreas tissue using GLP-2 Rabbit mAb (A5009, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (5)

Secondary Antibodies (26)