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GNE Rabbit pAb (A8570)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GNE Rabbit pAb (A8570)

Western blot analysis of various lysates using GNE Rabbit pAb (A8570) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GNE Rabbit pAb (A8570)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using GNE Rabbit pAb (A8570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GNE Rabbit pAb (A8570)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using GNE Rabbit pAb (A8570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameGNE Rabbit pAb
Catalog No.A8570
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GNE (NP_001121699.1).
SequenceMETYGYLQRESCFQGPHELYFKNLSKRNKQIMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALAL
Gene ID
Swiss Prot
SynonymsNM; DMRV; IBM2; Uae1; GLCNE; GNE
Calculated MW79kDa
Observed MW79kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesLO2, Jurkat, HeLa, mouse liver, rat liver
Cellular locationCytoplasm

Documents

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    ABclonal:Western blot - GNE Rabbit pAb (A8570)}

    Western blot - GNE Rabbit pAb (A8570)

    Western blot analysis of various lysates using GNE Rabbit pAb (A8570) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - GNE Rabbit pAb (A8570)}

    Immunohistochemistry - GNE Rabbit pAb (A8570)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using GNE Rabbit pAb (A8570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - GNE Rabbit pAb (A8570)}

    Immunohistochemistry - GNE Rabbit pAb (A8570)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus using GNE Rabbit pAb (A8570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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