제품 > 항체 > 다중클론항체(pAb)

GOLPH2 Rabbit pAb (A12584)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse large intestine, using GOLPH2 Rabbit pAb (A12584) at 1:9000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GOLPH2 Rabbit pAb (A12584)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GOLPH2 Rabbit pAb (A12584) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GOLPH2 Rabbit pAb (A12584)

Immunofluorescence analysis of MCF-7 cells using GOLPH2 Rabbit pAb (A12584). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using GOLPH2 Rabbit pAb (A12584) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product name	GOLPH2 Rabbit pAb
Catalog No.	A12584
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLPH2 (NP_057632.2).
Sequence	TGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL
Gene ID
Swiss Prot
Synonyms	GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
Calculated MW	45kDa
Observed MW	76kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse large intestine
Cellular location	Golgi apparatus, Single-pass type II membrane protein, cis-Golgi network membrane
Customer validation	WB(Homo sapiens)

Documents

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Research Area

GOLPH2 Rabbit pAb images

Western blot - GOLPH2 Rabbit pAb (A12584)

Immunohistochemistry - GOLPH2 Rabbit pAb (A12584)

Immunofluorescence - GOLPH2 Rabbit pAb (A12584)

Immunofluorescence analysis of MCF-7 cells using GOLPH2 Rabbit pAb (A12584). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence - GOLPH2 Rabbit pAb (A12584)

Confocal immunofluorescence analysis of Hela cells using GOLPH2 Rabbit pAb (A12584) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.