제품 > 항체 > 다중클론항체(pAb)

GPA5 Rabbit pAb (A19211)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - GPA5 Rabbit pAb (A19211)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GPA5 Rabbit pAb (A19211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameGPA5 Rabbit pAb
Catalog No.A19211
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
GPA5 is a membrane-associated protein capable of forming homodimers and that it is specifically localized to DVs in developing endosperm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 418-710 of Oryza sativa GPA5. (Q67WV5).
SequenceEATKKKDKEILHQAVLTEREKITQLQWDKDELYRKYSEMESNLKIEQNEKTRVQSEKTTASGEKEMLLEELETKRKEVESLQQHIGEFEAKSKADIKVLVKEVKSLRNSQKEMKKVLNQYHEEKTELERIVNREKQRSTRARFSREKILHECRLLRERLQECTAKFVADEQDTMTIDLSSLPDALDLVTTSDNRIRLLVAEAQLLSRDDEQGSSDDGDNSDGKSSVTMSSEDAYVTDEETTKMLSDLLIDNAQLRLRLNSLIRNAVNTAVKTEKEGSDGTVPKKTVLNWLLDR
Gene ID
Swiss Prot
SynonymsRab5a Effector; GPA5
Calculated MW80kDa
Observed MW120kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmilk grain
Cellular location
    ABclonal:Western blot - GPA5 Rabbit pAb (A19211)}

    Western blot - GPA5 Rabbit pAb (A19211)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GPA5 Rabbit pAb (A19211) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)