제품 > 항체 > 다중클론항체(pAb)

GPER1 Rabbit pAb (A10217)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GPER1 Rabbit pAb (A10217)

Western blot analysis of lysates from Mouse heart , using GPER1 Rabbit pAb (A10217) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of NIH/3T3 cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of U20S cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of C6 cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGPER1 Rabbit pAb
Catalog No.A10217
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3, 4, 5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 307-375 of human GPER1 (NP_001496.1).
SequenceHIVNLAAFSNSCLNPLIYSFLGETFRDKLRLYIEQKTNLPALNRFCHAALKAVIPDSTEQSDVRFSSAV
Gene ID
Swiss Prot
SynonymsmER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br; GPER1
Calculated MW42kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart
Cellular locationBasolateral cell membrane, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle membrane, Early endosome, Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Mitochondrion membrane, Multi-pass membrane protein, Nucleus, Recycling endosome, axon, cytoskeleton, dendrite, dendritic spine membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network.
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus)

IF(Danio rerio, Mus musculus)

IHC(Mus musculus)

WB(Mus musculus)

Other(Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GPER1 Rabbit pAb (A10217)}

Western blot - GPER1 Rabbit pAb (A10217)

Western blot analysis of lysates from Mouse heart , using GPER1 Rabbit pAb (A10217) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)}

Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of NIH/3T3 cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)}

Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of U20S cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GPER1 Rabbit pAb (A10217)}

Immunofluorescence - GPER1 Rabbit pAb (A10217)

Immunofluorescence analysis of C6 cells using GPER1 Rabbit pAb (A10217) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A10217? Please let us know so that we can cite the reference in this datasheet.