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GSDMC Rabbit mAb (A21213)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of various lysates using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from Rat stomach, using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from type (WT) and 293F cells transfected with GSDMC using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from HeLa cells, using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameGSDMC Rabbit mAb
Catalog No.A21213
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52482
Predicted to enable phosphatidylinositol-4, 5-bisphosphate binding activity; phosphatidylinositol-4-phosphate binding activity; and phosphatidylserine binding activity. Predicted to be involved in defense response to bacterium and pyroptosis. Predicted to act upstream of or within intestinal epithelial cell development. Located in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GSDMC (NP_113603.1).
SequenceMPSMLERISKNLVKEIGSKDLTPVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSSSVLETVVTGPFHFSDIMIQKHKADMGVNVGIEVSVSGEASVDHGCSLEFQIVTIPSPNLEDFQKRKLLDPEPSFLKECRR
Gene ID
Swiss Prot
SynonymsMLZE; GSDMC
Calculated MW58kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F transfected with GSDMC, HeLa, RAW264.7, Mouse stomach, Rat stomach
Cellular locationCytoplasm

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GSDMC Rabbit mAb (A21213)}

Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of various lysates using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - GSDMC Rabbit mAb (A21213)}

Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from Rat stomach, using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - GSDMC Rabbit mAb (A21213)}

Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from type (WT) and 293F cells transfected with GSDMC using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - GSDMC Rabbit mAb (A21213)}

Western blot - GSDMC Rabbit mAb (A21213)

Western blot analysis of lysates from HeLa cells, using GSDMC Rabbit mAb (A21213) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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