제품 > 항체 > 다중클론항체(pAb)

GSDMC Rabbit pAb (A14550)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - GSDMC Rabbit pAb (A14550)

Western blot analysis of various lysates, using GSDMC Rabbit pAb (A14550) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - GSDMC Rabbit pAb (A14550)

Immunofluorescence analysis of L929 cells using GSDMC Rabbit pAb (A14550) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGSDMC Rabbit pAb
Catalog No.A14550
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable phosphatidylinositol-4, 5-bisphosphate binding activity; phosphatidylinositol-4-phosphate binding activity; and phosphatidylserine binding activity. Predicted to be involved in defense response to bacterium and pyroptosis. Predicted to act upstream of or within intestinal epithelial cell development. Located in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human GSDMC (NP_113603.1).
SequenceMPSMLERISKNLVKEIGSKDLTPVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSSSVLETVVTGPFHFSDIMIQKHKADMGVNVG
Gene ID
Swiss Prot
SynonymsMLZE; GSDMC
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431
Cellular locationCytoplasm.
Customer validation

WB(Mus musculus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GSDMC Rabbit pAb (A14550)}

Western blot - GSDMC Rabbit pAb (A14550)

Western blot analysis of various lysates, using GSDMC Rabbit pAb (A14550) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - GSDMC Rabbit pAb (A14550)}

Immunofluorescence - GSDMC Rabbit pAb (A14550)

Immunofluorescence analysis of L929 cells using GSDMC Rabbit pAb (A14550) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14550? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)