제품 > 항체 > 단일클론항체(mAb)

GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from A-431 cells, using GSDMD Rabbit mAb (A20728) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit mAb (A20728) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Western blot analysis of lysates from PC-3 cells using GSDMD (Full length+C terminal) Rabbit mAb (A20728) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product name	GSDMD (Full length+C terminal) Rabbit mAb
Catalog No.	A20728
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC50993

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3).
Sequence	DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Gene ID
Swiss Prot
Synonyms	DF5L; DFNA5L; FKSG10; GSDMDC1; GSDMD
Calculated MW	53kDa
Observed MW	53kDa/22kDa/

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1 treated by PMA and LPS, A-431, PC-3
Cellular location	cytosol, extracellular region, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus, Ctenopharyngodon idellus, Sus scrofa, Other) IHC(Homo sapiens, Mus musculus, Rattus norvegicus) IF(Other) WB(Mus musculus, Rattus norvegicus) Co-IP(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구면역학 & 염증Inflammasome Signaling Pathway

GSDMD (Full length+C terminal) Rabbit mAb images

Western blot - GSDMD (Full length+C terminal) Rabbit mAb (A20728)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Cells

Application:

IF:

7.66

Species:

Mus musculus

PMID:

36429017

Title:

Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB Pathway - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Homo sapiens

PMID:

36762489

Title:

Latex Derived from Ficus carica L. Inhibited the Growth of NSCLC by Regulating the Caspase/Gasdermin/AKT Signaling Pathway

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Advanced biology

Application:

IF:

Species:

Homo sapiens

PMID:

37132155

Title:

Natural Oncolysis of Enterovirus 71 in Antitumor Therapy of Colorectal Cancer

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

The Kaohsiung journal of medical sciences

Application:

IF:

2.99

Species:

Mus musculus

PMID:

37092308

Title:

Bone marrow mesenchymal stem cell‐derived exosomes miR‐202‐5p inhibited pyroptosis to alleviate lung ischemic‐reperfusion injury by targeting CMPK2

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Journal of geriatric cardiology : JGC

Application:

IF:

3.18

Species:

Homo sapiens

PMID:

37397863

Title:

Identification of an LDLR variant in a Chinese familial hypercholesterolemia and its relation to ROS/NLRP3-Mediated pyroptosis in hepatic cells - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Journal of geriatric cardiology : JGC

Application:

IF:

3.18

Species:

Homo sapiens

PMID:

37397863

Title:

Identification of an LDLR variant in a Chinese familial hypercholesterolemia and its relation to ROS/NLRP3-Mediated pyroptosis in hepatic cells - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Rattus norvegicus

PMID:

37237918

Title:

Biochanin A Ameliorates Nephropathy in High-Fat Diet/Streptozotocin-Induced Diabetic Rats: Effects on NF-kB/NLRP3 Axis, Pyroptosis, and Fibrosis - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.62

Species:

Ctenopharyngodon idellus

PMID:

37805109

Title:

Resveratrol improves emamectin benzoate-induced pyroptosis and inflammation of Ctenopharyngodon idellus hepatic cells by alleviating oxidative stress

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Homo sapiens

PMID:

38056124

Title:

HgCl2 exposure mediates pyroptosis of HD11 cells and promotes M1 polarization and the release of inflammatory factors through ROS/Nrf2/NLRP3

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Sus scrofa

PMID:

37367489

Title:

Foot-and-Mouth Disease Virus Induces Porcine Gasdermin E-Mediated Pyroptosis through the Protease Activity of 3Cpro

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Sus scrofa

PMID:

38035519

Title:

Tanshinone IIA protects intestinal epithelial cells from deoxynivalenol-induced pyroptosis

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.29

Species:

Homo sapiens

PMID:

38214430

Title:

EEBR induces Caspase-1-dependent pyroptosis through the NF-B/NLRP3 signalling cascade in non-small cell lung cancer - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38544474

Title:

BRCC3 mediates inflammation and pyroptosis in cerebral ischemia/reperfusion injury by activating the NLRP6 inflammasome

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Chinese medicine

Application:

IHC

IF:

4.54

Species:

Homo sapiens

PMID:

36175965

Title:

Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Balkan medical journal

Application:

IF:

3.57

Species:

Mus musculus

PMID:

37350700

Title:

Exogenous Spermidine Alleviates Diabetic Myocardial Fibrosis Via Suppressing Inflammation and Pyroptosis in db/db Mice - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Fish & shellfish immunology

Application:

WB, IF

IF:

4.62

Species:

Other

PMID:

37536468

Title:

Co-exposure of avermectin and imidacloprid induces DNA damage, pyroptosis, and immune dysfunction in epithelioma papulosum cyprini cells via ROS-mediated

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Molecular medicine reports

Application:

IF:

3.4

Species:

Rattus norvegicus

PMID:

38639180

Title:

Dicoumarol attenuates NLRP3 inflammasome activation to inhibit inflammation and fibrosis in knee osteoarthritis - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

38552738

Title:

SLC25A3 negatively regulates NLRP3 inflammasome activation by restricting the function of NLRP3 - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

38479158

Title:

Dapagliflozin prevents kidney podocytes pyroptosis via miR-155-5p/HO-1/NLRP3 axis modulation

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

International immunopharmacology

Application:

IHC

IF:

5.6

Species:

Mus musculus

PMID:

38527402

Title:

Up-regulation of HSP90 in HDM-induced asthma causes pyroptosis of airway epithelial cells by activating the cGAS-STING-ER stress pathway

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Neurochemical research

Application:

IF:

4.4

Species:

Rattus norvegicus

PMID:

38834844

Title:

DL-3-n-Butylphthalide Ameliorates Post-stroke Emotional Disorders by Suppressing Neuroinflammation and PANoptosis

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

38902235

Title:

ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Immunity, inflammation and disease

Application:

IF:

3.2

Species:

Homo sapiens

PMID:

38869352

Title:

AIM2 inflammasome regulated by the IFN-/JAK2/STAT1 pathway promotes activation and pyroptosis of monocytes in Coronary Artery Disease - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IHC, WB

IF:

5.7

Species:

Mus musculus

PMID:

39134949

Title:

Multi-regulatory potency of USP1 on inflammasome components promotes pyroptosis in thyroid follicular cells and contributes to the progression of Hashimoto's thyroiditis - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Rattus norvegicus

PMID:

38930963

Title:

Oxymatrine Attenuates Ulcerative Colitis through Inhibiting Pyroptosis Mediated by the NLRP3 Inflammasome - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Diabetology & metabolic syndrome

Application:

IF:

4.8

Species:

Mus musculus,Rattus norvegicus

PMID:

38956716

Title:

Mesenchymal stem cells ameliorate inflammation and pyroptosis in diabetic cardiomyopathy via the miRNA-223-3p/NLRP3 pathway - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Scientific reports

Application:

IHC

IF:

4.6

Species:

Rattus norvegicus

PMID:

38782946

Title:

Semaglutide ameliorates pressure overload-induced cardiac hypertrophy by improving cardiac mitophagy to suppress the activation of NLRP3 inflammasome - PMC

References for GSDMD (Full length+C terminal) Rabbit mAb (A20728)

Product:

GSDMD (Full length+C terminal) Rabbit mAb

Journal:

Actas espanolas de psiquiatria

Application:

IF:

1.5

Species:

Homo sapiens

PMID:

39129701