제품 > 항체 > 단일클론항체(mAb)

Gasdermin D (Full Length+C terminal) Rabbit mAb (A23755)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb (A23755) at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb (A23755) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Overview

Product name	Gasdermin D (Full Length+C terminal) Rabbit mAb
Catalog No.	A23755
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61812

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human Gasdermin D(NP_079012.3)
Sequence	GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Gene ID
Swiss Prot
Synonyms	DF5L; DFNA5L; FKSG10; GSDMDC1; Gasdermin D (Full Length+C terminal)
Calculated MW	53KDa
Observed MW	23kDa/53kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:12000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1 treated by LPS and Nigericin, Recombination GSDMD-FL and N+C Protein
Cellular location	Cytoplasm, cytoso.