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[KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Western blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059)

Confocal imaging of THP-1 cells (treated with PMA and LPS) and THP-1 cells (untreated) cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	[KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb
Catalog No.	A24059
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57989

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Immunogen information

Immunogen	A synthetic peptide corresponding to residues surrounding Asp275 of human Gasdermin D(NP_079012.3).
Sequence	NFLTD
Gene ID
Swiss Prot
Synonyms	DF5L; DFNA5L; FKSG10; GSDMDC1
Calculated MW	53kDa
Observed MW	31kDa/

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:70000 - 1:280000 IF/ICC 1:100 - 1:400 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	THP-1, THP-1 treated by LPS and PMA, THP-1
Cellular location	cytosol, extracellular region, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane.
Customer validation	WB(Rattus norvegicus, Mus musculus) Other(Rattus norvegicus)