Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from HeLa cells, using Giantin Rabbit pAb (A22233) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
| Product name | Giantin Rabbit pAb |
|---|---|
| Catalog No. | A22233 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1123-1302 of human Giantin (NP_004478.3). |
|---|---|
| Sequence | QKLITSNTDASDGDSVALVKETVVISPPCTGSSEHWKPELEEKILALEKEKEQLQKKLQEALTSRKAILKKAQEKERHLREELKQQKDDYNRLQEQFDEQSKENENIGDQLRQLQIQVRESIDGKLPSTDQQESCSSTPGLEEPLFKATEQHHTQPVLESNLCPDWPSHSEDASALQGGT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | GCP; GCP372; GOLIM1; Giantin |
| Calculated MW | 376kDa |
| Observed MW | 376kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | HeLa |
| Cellular location | cis-Golgi network, cytosol, endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Golgi stack |
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* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1715736628 "ABclonal:Western blot - Giantin Rabbit pAb (A22233)")
_IF_01.jpg?t=1715736628 "ABclonal:Immunofluorescence - Giantin Rabbit pAb (A22233)")
