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Grp94 Rabbit pAb (A6272)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Grp94 Rabbit pAb (A6272)

Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Grp94 Rabbit pAb (A6272)

Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Grp94 Rabbit pAb (A6272)

Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Grp94 Rabbit pAb (A6272)

Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Grp94 Rabbit pAb (A6272)

Immunofluorescence analysis of NIH-3T3 cells using Grp94 Rabbit pAb (A6272) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGrp94 Rabbit pAb
Catalog No.A6272
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 501-750 of human Grp94 (NP_003290.1).
SequenceSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNID
Gene ID
Swiss Prot
SynonymsECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m; Grp94
Calculated MW92kDa
Observed MW100kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, NIH/3T3, PC-12, Rat testis, PC-12, Rat testis, MCF7, NIH/3T3
Cellular locationEndoplasmic reticulum lumen, Melanosome.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Grp94 Rabbit pAb (A6272)}

    Western blot - Grp94 Rabbit pAb (A6272)

    Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Grp94 Rabbit pAb (A6272)}

    Western blot - Grp94 Rabbit pAb (A6272)

    Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Grp94 Rabbit pAb (A6272)}

    Western blot - Grp94 Rabbit pAb (A6272)

    Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Grp94 Rabbit pAb (A6272)}

    Western blot - Grp94 Rabbit pAb (A6272)

    Western blot analysis of various lysates using Grp94 Rabbit pAb (A6272) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)}

    Immunohistochemistry - Grp94 Rabbit pAb (A6272)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)}

    Immunohistochemistry - Grp94 Rabbit pAb (A6272)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Grp94 Rabbit pAb (A6272)}

    Immunohistochemistry - Grp94 Rabbit pAb (A6272)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using Grp94 Rabbit pAb (A6272) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Grp94 Rabbit pAb (A6272)}

    Immunofluorescence - Grp94 Rabbit pAb (A6272)

    Immunofluorescence analysis of NIH-3T3 cells using Grp94 Rabbit pAb (A6272) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (2)

    ELISA 키트 (1)

    Secondary Antibodies (26)