제품 > 항체 > 단일클론항체(mAb)

HCoV-OC43 Spike S1 Rabbit mAb (A22739)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using HCoV-OC43 Spike S1 Rabbit mAb (A22739) at 1:8000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	HCoV-OC43 Spike S1 Rabbit mAb
Catalog No.	A22739
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57814

Background

S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Immunogen information

Immunogen	A synthesized peptide derived from human HCoV-OC43 Spike S1.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW	150kDa
Observed MW	110kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:9000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag)
Cellular location