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HCoV-OC43 Spike S2 Rabbit pAb (A20616)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:HCoV-OC43

ABclonal:Western blot - HCoV-OC43 Spike S2 Rabbit pAb (A20616)

Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag), using HCoV-OC43 Spike S2 Rabbit pAb (A20616) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameHCoV-OC43 Spike S2 Rabbit pAb
Catalog No.A20616
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 782-881 of coronavirus Spike S2 (YP_009555241.1).
SequenceEPVGGLYEIQIPSEFTIGNMVEFIQTSSPKVTIDCAAFVCGDYAACKSQLVEYGSFCDNINAILTEVNELLDTTQLQVANSLMNGVTLSTKLKDGVNFNV
Gene ID
Swiss Prot
Synonyms
Calculated MW150kDa
Observed MW75kDa/145kDa
ReactivityHCoV-OC43
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag)
Cellular location

Documents

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Lot number

    ABclonal:Western blot - HCoV-OC43 Spike S2 Rabbit pAb (A20616)}

    Western blot - HCoV-OC43 Spike S2 Rabbit pAb (A20616)

    Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag), using HCoV-OC43 Spike S2 Rabbit pAb (A20616) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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