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HDAC9 Rabbit mAb (A2226)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - HDAC9 Rabbit mAb (A2226)

Western blot analysis of various lysates, using HDAC9 Rabbit mAb (A2226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - HDAC9 Rabbit mAb (A2226)

Immunohistochemistry analysis of paraffin-embedded rat testis using HDAC9 Rabbit mAb (A2226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC9 Rabbit mAb (A2226)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HDAC9 Rabbit mAb (A2226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HDAC9 Rabbit mAb (A2226)

Confocal imaging of U-2 OS cells using HDAC9 Rabbit mAb (A2226, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - HDAC9 Rabbit mAb (A2226)

Confocal imaging of K-562 cells using HDAC9 Rabbit mAb (A2226, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameHDAC9 Rabbit mAb
Catalog No.A2226
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0735
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC9 (Q9UKV0).
SequenceMHSMISSVDVKSEVPVGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKELLAIKQQQELL
Gene ID
Swiss Prot
SynonymsHD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDAC9
Calculated MW111kDa
Observed MW150kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRD, K-562
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Mus musculus)

IP(Arabidopsis thaliana)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - HDAC9 Rabbit mAb (A2226)}

Western blot - HDAC9 Rabbit mAb (A2226)

Western blot analysis of various lysates, using HDAC9 Rabbit mAb (A2226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - HDAC9 Rabbit mAb (A2226)}

Immunohistochemistry - HDAC9 Rabbit mAb (A2226)

Immunohistochemistry analysis of paraffin-embedded rat testis using HDAC9 Rabbit mAb (A2226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC9 Rabbit mAb (A2226)}

Immunohistochemistry - HDAC9 Rabbit mAb (A2226)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HDAC9 Rabbit mAb (A2226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - HDAC9 Rabbit mAb (A2226)}

Immunofluorescence - HDAC9 Rabbit mAb (A2226)

Confocal imaging of U-2 OS cells using HDAC9 Rabbit mAb (A2226, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - HDAC9 Rabbit mAb (A2226)}

Immunofluorescence - HDAC9 Rabbit mAb (A2226)

Confocal imaging of K-562 cells using HDAC9 Rabbit mAb (A2226, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2226? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)