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HDAC9 Rabbit pAb (A1516)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HDAC9 Rabbit pAb (A1516)

Western blot analysis of various lysates using HDAC9 Rabbit pAb (A1516) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded human placenta using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded rat lung using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameHDAC9 Rabbit pAb
Catalog No.A1516
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC9 (NP_478056.1).
SequenceMHSMISSVDVKSEVPVGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKELLAIKQQQELL
Gene ID
Swiss Prot
SynonymsHD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDAC9
Calculated MW111kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRD, K-562
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - HDAC9 Rabbit pAb (A1516)}

Western blot - HDAC9 Rabbit pAb (A1516)

Western blot analysis of various lysates using HDAC9 Rabbit pAb (A1516) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)}

Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded human placenta using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)}

Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - HDAC9 Rabbit pAb (A1516)}

Immunohistochemistry - HDAC9 Rabbit pAb (A1516)

Immunohistochemistry analysis of paraffin-embedded rat lung using HDAC9 Rabbit pAb (A1516) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1516? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)