제품 > 항체 > 다중클론항체(pAb)

HES1 Rabbit pAb (A11718)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using HES1 Rabbit pAb (A11718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review

Overview

Product name	HES1 Rabbit pAb
Catalog No.	A11718
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 90-165 of human HES1 (NP_005515.1).
Sequence	NLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLANCMTQINAMTYPGQPHPALQA
Gene ID
Swiss Prot
Synonyms	HHL; HRY; HES-1; bHLHb39; HES1
Calculated MW	30kDa
Observed MW	29kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	MCF7, SKOV3
Cellular location	Nucleus.
Customer validation	(Mouse spleen cells) WB(Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Homo sapiens, Oreochromis niloticus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구후성유전학 & 핵 신호전달전사 인자

HES1 Rabbit pAb images

Western blot - HES1 Rabbit pAb (A11718)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Acta Histochemica

Application:

IF:

2.3

Species:

Homo sapiens

PMID:

32113857

Title:

miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

J Cancer

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

32913469

Title:

PM2. 5 downregulates MicroRNA-139-5p and induces EMT in Bronchiolar Epithelium Cells by targeting Notch1

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Nutr Metab (Lond)

Application:

IF:

4.7

Species:

Homo sapiens

PMID:

33964955

Title:

Combined effect of vitamin C and vitamin D 3 on intestinal epithelial barrier by regulating Notch signaling pathway

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Sci Prog

Application:

IF:

2.6

Species:

Rattus norvegicus

PMID:

34231445

Title:

Effects of serum starvation and vascular endothelial growth factor stimulation on the expression of Notch signalling pathway components

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

35281083

Title:

miR-381-3p Cooperated With Hes1 to Regulate the Proliferation and Differentiation of Retinal Progenitor Cells

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Cellular signalling

Application:

IHC

IF:

4.4

Species:

Homo sapiens

PMID:

37730102

Title:

ZNF746 promotes M2 macrophage polarisation and favours tumour progression in breast cancer via the Jagged1/Notch pathway

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Molecular and cellular biochemistry

Application:

WB, IHC

IF:

3.5

Species:

Mus musculus

PMID:

28744818

Title:

Epigenetic modification of TLE1 induce abnormal differentiation in diabetic mice intestinal epithelium

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.4

Species:

Mus musculus

PMID:

37406788

Title:

Downregulation of a potential therapeutic target NPAS2, regulated by p53, alleviates pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Apoptosis : an international journal on programmed cell death

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38553613

Title:

Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Acta biomaterialia

Application:

IF:

9.4

Species:

Homo sapiens

PMID:

38692468

Title:

Understanding the multi-functionality and tissue-specificity of decellularized dental pulp matrix hydrogels for endodontic regeneration

References for HES1 Rabbit pAb (A11718)

Product:

HES1 Rabbit pAb

Journal:

Journal of fish diseases

Application:

IHC

IF:

2.2

Species:

Oreochromis niloticus

PMID:

35758189

Title:

Establishment of an astrocytelike cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the bloodbrain barrier