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HLA-DQA1 Rabbit mAb (A11252)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HLA-DQA1 Rabbit mAb (A11252)

Western blot analysis of various lysates using HLA-DQA1 Rabbit mAb (A11252) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameHLA-DQA1 Rabbit mAb
Catalog No.A11252
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0564
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 155-254 of human HLA-DQA1 (P01909).
SequenceEGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSELTETVVCALGLSVGLVGIVVGTVFIIRGLRSVGASRHQGPL
Gene ID
Swiss Prot
SynonymsDQA1; DQ-A1; CELIAC1; HLA-DQA; HLA-DQB1; HLA-DQA1*; HLA-DQA1
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, Mouse lung, Mouse spleen, Rat lung, Rat spleen
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - HLA-DQA1 Rabbit mAb (A11252)}

    Western blot - HLA-DQA1 Rabbit mAb (A11252)

    Western blot analysis of various lysates using HLA-DQA1 Rabbit mAb (A11252) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)}

    Immunohistochemistry - HLA-DQA1 Rabbit mAb (A11252)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HLA-DQA1 Rabbit mAb (A11252) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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