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HLA-DQA1 Rabbit pAb (A2168)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - HLA-DQA1 Rabbit pAb (A2168)

Western blot analysis of various lysates using HLA-DQA1 Rabbit pAb (A2168) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)

Immunohistochemistry analysis of paraffin-embedded human prostate using HLA-DQA1 Rabbit pAb (A2168) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)

Immunohistochemistry analysis of paraffin-embedded human esophagus using HLA-DQA1 Rabbit pAb (A2168) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameHLA-DQA1 Rabbit pAb
Catalog No.A2168
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-216 of human HLA-DQA1 (NP_002113.2).
SequenceEDIVADHVASCGVNLYQFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPEFSKFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAATNEVPEVTVFSKSPVTLGQPNTLICLVDNIFPPVVNITWLSNGQSVTEGVSETSFLSKSDHSFFKISYLTFLPSADEIYDCKVEHWGLDQPLLKHWEPEIPAPMSELT
Gene ID
Swiss Prot
SynonymsDQA1; DQ-A1; CELIAC1; HLA-DQA; HLA-DQB1; HLA-DQA1*; HLA-DQA1
Calculated MW28kDa
Observed MW28kDaa-37kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW620, HT-29, Lovo, Mouse brain, Mouse stomach
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HLA-DQA1 Rabbit pAb (A2168)}

    Western blot - HLA-DQA1 Rabbit pAb (A2168)

    Western blot analysis of various lysates using HLA-DQA1 Rabbit pAb (A2168) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)}

    Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)

    Immunohistochemistry analysis of paraffin-embedded human prostate using HLA-DQA1 Rabbit pAb (A2168) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)}

    Immunohistochemistry - HLA-DQA1 Rabbit pAb (A2168)

    Immunohistochemistry analysis of paraffin-embedded human esophagus using HLA-DQA1 Rabbit pAb (A2168) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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