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HSPB8/HSP22 Rabbit mAb (A13518)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A13518)

Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (A13518) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)

Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb (A13518) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)

Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (A13518) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameHSPB8/HSP22 Rabbit mAb
Catalog No.A13518
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0715
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPB8/HSP22 (Q9UJY1).
SequenceMADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCV
Gene ID
Swiss Prot
SynonymsH11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22; CRYAC
Calculated MW22kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart, Rat heart
Cellular locationCytoplasm, Nucleus

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    ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A13518)}

    Western blot - HSPB8/HSP22 Rabbit mAb (A13518)

    Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (A13518) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)}

    Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)

    Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb (A13518) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)}

    Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A13518)

    Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (A13518) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)