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HSPB8/HSP22 Rabbit mAb (A25396)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A25396)

Western blot analysis of lysates from COS-7 cells using HSPB8/HSP22 Rabbit mAb (A25396) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A25396)

Western blot analysis of various lysates using HSPB8/HSP22 Rabbit mAb (A25396) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HL-60.
Exposure time: 10s.

ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

Confocal imaging of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

Confocal imaging of paraffin-embedded Mouse heart tissue using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

Confocal imaging of RD cells using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameHSPB8/HSP22 Rabbit mAb
Catalog No.A25396
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66379
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8/HSP22 (NP_055180.1).
SequenceMADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT
Gene ID
Swiss Prot
SynonymsH11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
Calculated MW22kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:30000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesCOS-7, RD, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle
Cellular locationCytoplasm, Nucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A25396)}

    Western blot - HSPB8/HSP22 Rabbit mAb (A25396)

    Western blot analysis of lysates from COS-7 cells using HSPB8/HSP22 Rabbit mAb (A25396) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Western blot - HSPB8/HSP22 Rabbit mAb (A25396)}

    Western blot - HSPB8/HSP22 Rabbit mAb (A25396)

    Western blot analysis of various lysates using HSPB8/HSP22 Rabbit mAb (A25396) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): HL-60.
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

    Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

    Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunohistochemistry - HSPB8/HSP22 Rabbit mAb (A25396)

    Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle tissue using HSPB8/HSP22 Rabbit mAb (A25396) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

    Confocal imaging of U-2 OS cells using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

    Confocal imaging of paraffin-embedded Mouse heart tissue using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)}

    Immunofluorescence - HSPB8/HSP22 Rabbit mAb (A25396)

    Confocal imaging of RD cells using HSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)