제품 > 항체 > 단일클론항체(mAb)

IκBα Rabbit mAb (A24909)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IκBα Rabbit mAb (A24909)

Western blot analysis of various lysates, using IκBα Rabbit mAb (A24909) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)

Confocal imaging of HeLa cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)

Confocal imaging of Hep G2 cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)

Confocal imaging of NIH/3T3 cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameIκBα Rabbit mAb
Catalog No.A24909
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63567
Enables NF-kappaB binding activity and heat shock protein binding activity. Involved in several processes, including cytoplasmic sequestering of NF-kappaB; leukocyte migration involved in inflammatory response; and liver regeneration. Acts upstream of or within cellular response to cytokine stimulus and cellular response to organic cyclic compound. Located in cytosol and nucleus. Part of protein-containing complex. Used to study gastritis; pulmonary emphysema; and sciatic neuropathy. Biomarker of several diseases, including acute necrotizing pancreatitis; alcoholic hepatitis; artery disease (multiple); middle cerebral artery infarction; and toxic shock syndrome. Human ortholog(s) of this gene implicated in several diseases, including childhood-onset asthma; hematologic cancer (multiple); hepatitis B; hepatitis C; and lung disease (multiple). Orthologous to human NFKBIA (NFKB inhibitor alpha).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-314 of Rat IκBα(NP_001099190.2).
SequenceMFQPAGHGQDWAMEGPRDGLKKERLVDDRHDSGLDSMKDEDYEQMVKELREIRLQPQEAPLAAEPWKQQLTEDGDSFLHLAIIHEEKTLTMEVIGQVKGDLAFLNFQNNLQQTPLHLAVITNQPGIAEALLKAGCDPELRDFRGNTPLHLACEQGCLASVAVLTQTCTPQHLHSVLQATNYNGHTCLHLASIHGYLGIVEHLVTLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQTLPESEDEESYDTESEFTEDELPYDDCVFGGQRLTL
Gene ID
Swiss Prot
SynonymsRL/IF-1; IκBα
Calculated MW35kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:7000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse thymus, Mouse liver (Low expression control)
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - IκBα Rabbit mAb (A24909)}

    Western blot - IκBα Rabbit mAb (A24909)

    Western blot analysis of various lysates, using IκBα Rabbit mAb (A24909) at 1:6000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)}

    Immunofluorescence - IκBα Rabbit mAb (A24909)

    Confocal imaging of HeLa cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)}

    Immunofluorescence - IκBα Rabbit mAb (A24909)

    Confocal imaging of Hep G2 cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - IκBα Rabbit mAb (A24909)}

    Immunofluorescence - IκBα Rabbit mAb (A24909)

    Confocal imaging of NIH/3T3 cells using IκBα Rabbit mAb (A24909, at dilution of 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:100) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A24909? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)