제품 > 항체 > 다중클론항체(pAb)

IFN-beta Rabbit pAb (A23651)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - IFN-beta Rabbit pAb (A23651)

Western blot analysis of Recombinant Mouse IFN-beta Protein (RP01076) using IFN-beta Rabbit pAb (A23651) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng/5ng/1ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - IFN-beta Rabbit pAb (A23651)

Immunofluorescence analysis of HepG2 cells using IFN-beta Rabbit pAb (A23651) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IFN-beta Rabbit pAb (A23651)

Immunofluorescence analysis of paraffin-embedded mouse small intestine using IFN-beta Rabbit pAb (A23651) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIFN-beta Rabbit pAb
Catalog No.A23651
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1)
SequenceMNNRWILHAAFLLCFSTTALSINYKQLQLQERTNIRKCQELLEQLNGKINLTYRADFKIPMEMTEKMQKSYTAFAIQEMLQNVFLVFRNNFSSTGWNETIVVRLLDELHQQTVFLKTVLEEKQEERLTWEMSSTALHLKSYYWRVQRYLKLMKYNSYAWMVVRAEIFRNFLIIRRLTRNFQN
Gene ID
Swiss Prot
SynonymsIfb; IFNB; If1da1; IFN-beta
Calculated MW22kDa
Observed MW63kDa (C-hFc&His taged Active Recombinant Mouse IFN-beta Protein),24kDa/26kDa(Endogenous)
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRecombinant Mouse IFN-beta Protein
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - IFN-beta Rabbit pAb (A23651)}

    Western blot - IFN-beta Rabbit pAb (A23651)

    Western blot analysis of Recombinant Mouse IFN-beta Protein (RP01076) using IFN-beta Rabbit pAb (A23651) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 10ng/5ng/1ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunofluorescence - IFN-beta Rabbit pAb (A23651)}

    Immunofluorescence - IFN-beta Rabbit pAb (A23651)

    Immunofluorescence analysis of HepG2 cells using IFN-beta Rabbit pAb (A23651) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IFN-beta Rabbit pAb (A23651)}

    Immunofluorescence - IFN-beta Rabbit pAb (A23651)

    Immunofluorescence analysis of paraffin-embedded mouse small intestine using IFN-beta Rabbit pAb (A23651) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    ELISA 키트 (1)

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    Secondary Antibodies (26)