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IGF2BP3/IMP3 Rabbit mAb (A23295)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using IGF2BP3/IMP3 Rabbit mAb (A23295) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - IGF2BP3/IMP3 Rabbit mAb (A23295)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using IGF2BP3/IMP3 Rabbit mAb (A23295) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using IGF2BP3/IMP3 Rabbit mAb (A23295) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using IGF2BP3/IMP3 Rabbit mAb (A23295) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - IGF2BP3/IMP3 Rabbit mAb (A23295)

Immunofluorescence analysis of paraffin-embedded human placenta using IGF2BP3/IMP3 Rabbit mAb (A23295) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - IGF2BP3/IMP3 Rabbit mAb (A23295)

Immunoprecipitation analysis of 3 μg extracts of SH-SY5Y cells using 300ug IGF2BP3/IMP3 Rabbit mAb (A23295). Western blot was performed from the immunoprecipitate using IGF2BP3/IMP3 Rabbit mAb (A23295) at a dilition of 1:2000.

Overview

Product name	IGF2BP3/IMP3 Rabbit mAb
Catalog No.	A23295
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60319

Background

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 300-579 of human IGF2BP3/IMP3（NP_006538.2）.
Sequence	NKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPC
Gene ID
Swiss Prot
Synonyms	KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; IGF2BP3/IMP3
Calculated MW	64kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:8000 IHC-P 1:200 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	Jurkat, T-47D(Negative control), NIH/3T3, Mouse testis
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus)