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IGF2BP3 Rabbit pAb (A4444)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - IGF2BP3 Rabbit pAb (A4444)

Western blot analysis of various lysates using IGF2BP3 Rabbit pAb (A4444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded human esophagus using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded human stomach using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded mouse lung using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IGF2BP3 Rabbit pAb (A4444)

Immunofluorescence analysis of U2OS cells using IGF2BP3 Rabbit pAb (A4444) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - IGF2BP3 Rabbit pAb (A4444)

Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg IGF2BP3 antibody (A4444). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (A4444) at a dilution of 1:1000.

Overview

Product nameIGF2BP3 Rabbit pAb
Catalog No.A4444
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-579 of human IGF2BP3 (NP_006538.2).
SequenceKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK
Gene ID
Swiss Prot
SynonymsKOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; IGF2BP3
Calculated MW64kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHepG2, A-549, 293T, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

IHC(Homo sapiens)

WB(Gallus gallus, Homo sapiens, Pampus argenteus)

IHC(Pampus argenteus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IGF2BP3 Rabbit pAb (A4444)}

Western blot - IGF2BP3 Rabbit pAb (A4444)

Western blot analysis of various lysates using IGF2BP3 Rabbit pAb (A4444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)}

Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded human esophagus using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)}

Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded human stomach using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)}

Immunohistochemistry - IGF2BP3 Rabbit pAb (A4444)

Immunohistochemistry analysis of paraffin-embedded mouse lung using IGF2BP3 Rabbit pAb (A4444) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IGF2BP3 Rabbit pAb (A4444)}

Immunofluorescence - IGF2BP3 Rabbit pAb (A4444)

Immunofluorescence analysis of U2OS cells using IGF2BP3 Rabbit pAb (A4444) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - IGF2BP3 Rabbit pAb (A4444)}

Immunoprecipitation - IGF2BP3 Rabbit pAb (A4444)

Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg IGF2BP3 antibody (A4444). Western blot was performed from the immunoprecipitate using IGF2BP3 antibody (A4444) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)