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IKKε Rabbit pAb (A14093)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IKKε Rabbit pAb (A14093)

Western blot analysis of various lysates using IKKε Rabbit pAb (A14093) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - IKKε Rabbit pAb (A14093)

Immunohistochemistry analysis of paraffin-embedded rat spleen using IKKε Rabbit pAb (A14093) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IKKε Rabbit pAb (A14093)

Immunohistochemistry analysis of paraffin-embedded mouse brain using IKKε Rabbit pAb (A14093) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameIKKε Rabbit pAb
Catalog No.A14093
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKKε (NP_054721.1).
SequenceLRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV
Gene ID
Swiss Prot
SynonymsIKKE; IKKI; IKK-E; IKK-i; IKKε
Calculated MW80kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, 293T, SW480, Mouse liver
Cellular locationCytoplasm, Nucleus, PML body

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ABclonal:Western blot - IKKε Rabbit pAb (A14093)}

Western blot - IKKε Rabbit pAb (A14093)

Western blot analysis of various lysates using IKKε Rabbit pAb (A14093) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - IKKε Rabbit pAb (A14093)}

Immunohistochemistry - IKKε Rabbit pAb (A14093)

Immunohistochemistry analysis of paraffin-embedded rat spleen using IKKε Rabbit pAb (A14093) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IKKε Rabbit pAb (A14093)}

Immunohistochemistry - IKKε Rabbit pAb (A14093)

Immunohistochemistry analysis of paraffin-embedded mouse brain using IKKε Rabbit pAb (A14093) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)