제품 > 항체 > 단일클론항체(mAb)

IL10 Rabbit mAb (A12255)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using IL10 Rabbit mAb (A12255) at 1:1000 dilution incubated overnight at 4°C. THP-1 cells were treated by LPS (100ng/ml) and Brefeldin A (300ng/ml) for different times of stimulation.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from Rat thymus, using IL10 Rabbit mAb (A12255) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - IL10 Rabbit mAb (A12255)

Confocal imaging of HeLa cells using IL10 Rabbit mAb (A12255, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	IL10 Rabbit mAb
Catalog No.	A12255
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0650

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IL10 (P22301).
Sequence	MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAEN
Gene ID
Swiss Prot
Synonyms	CSIF; TGIF; GVHDS; IL-10; IL10A; IL10
Calculated MW	21kDa
Observed MW	18kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	THP-1 treated by LPS and Brefeldin A, Mouse spleen, Rat thymus
Cellular location	extracellular region, extracellular space.
Customer validation	IF(Rattus norvegicus, Other, Homo sapiens, Mus musculus) IHC(Gallus gallus, Rattus norvegicus) WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

IL10 Rabbit mAb images

Western blot - IL10 Rabbit mAb (A12255)

Immunofluorescence - IL10 Rabbit mAb (A12255)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Biomaterials

Application:

IF:

12.47

Species:

Rattus norvegicus

PMID:

34894585

Title:

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IHC

IF:

3.32

Species:

Gallus gallus

PMID:

36113179

Title:

2.45 GHz microwave radiation induced oxidative stress: Role of inflammatory cytokines in regulating male fertility through estrogen receptor alpha in Gallus gallus …

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Acta pharmacologica Sinica

Application:

IF:

7.16

Species:

Mus musculus

PMID:

35501362

Title:

Novel Caspase-1 inhibitor CZL80 improves neurological function in mice after progressive ischemic stroke within a long therapeutic time-window

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.58

Species:

Rattus norvegicus

PMID:

35658977

Title:

DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Gut microbes

Application:

IF:

9.43

Species:

Mus musculus

PMID:

36398889

Title:

Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206+ macrophagesIL-10 activation - PMC

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Metabolic brain disease

Application:

IF:

3.65

Species:

Mus musculus

PMID:

37470879

Title:

Wuzi Yanzong Pill relieves MPTP-induced motor dysfunction and neuron loss by inhibiting NLRP3 inflammasome-mediated neuroinflammation

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Journal of affective disorders

Application:

IF:

6.53

Species:

Mus musculus

PMID:

37634821

Title:

Effects of aerobic exercise on depression-like behavior and TLR4/NLRP3 pathway in hippocampus CA1 region of CUMS-depressed mice

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Molecular & cellular proteomics : MCP

Application:

IF:

Species:

Homo sapiens

PMID:

37532176

Title:

The Hyperproliferation Mechanism of Cholesteatoma Based on Proteomics: SNCA Promotes Autophagy-Mediated Cell Proliferation through the PI3K/AKT

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Homo sapiens, Mus musculus

PMID:

37673366

Title:

Edaravone dexborneol ameliorates cognitive impairment by regulating the NF-B pathway through AHR and promoting microglial polarization towards the M2

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Science advances

Application:

IF:

14.95

Species:

Rattus norvegicus

PMID:

38117891

Title:

An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment - PMC

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Tissue & cell

Application:

IF:

2.58

Species:

Homo sapiens

PMID:

38134573

Title:

Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Applied bionics and biomechanics

Application:

IF:

1.66

Species:

Rattus norvegicus

PMID:

35528532

Title:

The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Probiotics and antimicrobial proteins

Application:

IF:

4.9

Species:

Other

PMID:

38170389

Title:

Impact of Dietary Probiotics on the Immune and Reproductive Physiology of Pubertal Male Japanese Quail (Coturnix coturnix japonica) Administered at the Onset of

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Drug design, development and therapy

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38651136

Title:

Obacunone Alleviates Inflammatory Pain by Promoting M2 Microglial Polarization and by Activating Nrf2/HO-1 Signaling Pathway - PMC

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.2

Species:

Homo sapiens

PMID:

38679256

Title:

Dual-crosslinking gelatin-hyaluronic acid methacrylate based biomimetic PDAC desmoplastic niche enhances tumor-associated macrophages recruitment and M2

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Mus musculus

PMID:

37635177

Title:

InflammationResponsive Hydrogel Spray for Synergistic Prevention of Traumatic Heterotopic Ossification via DualHomeostatic Modulation Strategy

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Transplant immunology

Application:

IHC

IF:

1.5

Species:

Rattus norvegicus

PMID:

38815767

Title:

Transcriptomics-based exploration of shared M1-type macrophage-related biomarker in acute kidney injury after kidney transplantation and acute rejection after

References for IL10 Rabbit mAb (A12255)

Product:

IL10 Rabbit mAb

Journal:

Acta biomaterialia

Application:

IF:

9.7

Species:

Rattus norvegicus

PMID:

38761961