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IP3R Rabbit mAb (A23760)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IP3R Rabbit mAb (A23760)

Western blot analysis of various lysates, using IP3R Rabbit mAb (A23760) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - IP3R Rabbit mAb (A23760)

Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using IP3R Rabbit mAb (A23760) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IP3R Rabbit mAb (A23760)

Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat brain using IP3R Rabbit mAb (A23760) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - IP3R Rabbit mAb (A23760)

Immunoprecipitation analysis of 300ug extracts from SH-SY5Y cells using 3ug IP3R Rabbit mAb (A23760). Western blot was performed from the immunoprecipitate using IP3R Rabbit mAb (A23760) at a dilition of 1:10000.

Overview

Product nameIP3R Rabbit mAb
Catalog No.A23760
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61062
This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1820-1920 of human IP3R. (NP_001365381.1).
SequenceIMNASSDRVFHESILLAIALLEGGNTTIQHSFFCRLTEDKKSEKFFKVFYDRMKVAQQEIKATVTVNTSDLGNKKKDDEVDRDAPSRKKAKEPTTQITEEV
Gene ID
Swiss Prot
SynonymsITPR1; ACV; CLA4; INSP3R1; IP3R; IP3R1; PPP1R94; SCA15; SCA16; SCA29; inositol 1; 4; 5-trisphosphate receptor type 1
Calculated MW306-313kDa
Observed MW310kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSH-SY5Y
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein.

Documents

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ABclonal:Western blot - IP3R Rabbit mAb (A23760)}

Western blot - IP3R Rabbit mAb (A23760)

Western blot analysis of various lysates, using IP3R Rabbit mAb (A23760) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)}

Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)}

Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IP3R Rabbit mAb (A23760)}

Immunohistochemistry - IP3R Rabbit mAb (A23760)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using IP3R Rabbit mAb (A23760) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - IP3R Rabbit mAb (A23760)}

Immunofluorescence - IP3R Rabbit mAb (A23760)

Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse brain using IP3R Rabbit mAb (A23760) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IP3R Rabbit mAb (A23760)}

Immunofluorescence - IP3R Rabbit mAb (A23760)

Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat brain using IP3R Rabbit mAb (A23760) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - IP3R Rabbit mAb (A23760)}

Immunoprecipitation - IP3R Rabbit mAb (A23760)

Immunoprecipitation analysis of 300ug extracts from SH-SY5Y cells using 3ug IP3R Rabbit mAb (A23760). Western blot was performed from the immunoprecipitate using IP3R Rabbit mAb (A23760) at a dilition of 1:10000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)